ProductDescriptionSize:100μlAbType:PolyclonalReactivity:AllImmunogen:Methylated-KLHHost:Rabbit

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ProductDescriptionTherabbitpolyclonalantibodywasproducedbyimmunizinganimalswithmethylated-KLHconjugates.AntibodywaspurifiedwithproteinA-conjugatedagarosefollowedbymethyllysineagaroseaffinitychromatography.SpecificityThepananti-mono-anddimethyllysineantibodydetectsproteinspost-translationallymodifiedbymono-anddimethylationonlysineresidues,butnotthetrimethyllysine,acetyllysine,orunmodifiedlysineresiduesinawiderangeofsequencecontexts.RecommendedApplicationsELISA,WB,IAP*WB=Westernblotting;IAP=Immunoaffinitypreciptation.RecommendedDilutionWB:1:1000;IAP:1:200.IMPORTANTNOTESForWesternblotting,incubatemembranewithdilutedantibodyin5%BSA,1xTBS,0.1%Tween-20at4℃withgentleshaking,overnight.Optimaldilutions/concentrationsshouldbedeterminedbytheenduserandmaybeassaydependent.

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A.Dotblottinganalysisonindicatedamountoflysinemethylated-KLHconjugates(Lane1),trimethylated-KLHconjugates(Lane2),acetylated-KLHconjugates(Lane3),andunmodifiedKLH(Lane4)usinganti-mono-,dimethyllysinepolyclonalantibody.B.Westernblottinganalysison20µgofcrudeproteinsfromHeLawholecelllysatesusinganti-mono-anddimethyllysinepolyclonalantibody(duplicatedloADIng,1:2000).

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ScientificDescriptionThepost-translationalε-aminolysinemethylationofproteinsisanimportantreversIBLemodificationcontrollingproteinactivity.Thereversiblelysinemethylationofproteinsplaysavitalroleintheregulationofmanycellularprocessesincludingchromatinremodeling,geneexpression,cellproliferation,etc.Mono-anddimethylationaremethylatedformsoflysineandoccurinbothhistonesandnon-histonesubstrates.Methylationoflysineresiduesismodulatedbyspecificcounteractiveenzymesincludinglysinemethylases(KMTs),demethylases(KDMs),histonemethylases(HMTs),andhistonedemethylases(HDMs),whosepathwayshavebecomepromisingtargetsfordiscoveryofanti-cancerdrugs.Storage&StABIlityTheantibodyisdeliveredinlyophilizedpowder.Uponreceipt,pleasecentrifugetheantibodyat12,000xgfor20seconds,andthenreconstituteitwiththeantibodystabilizerprovided.ThestabilizercontainsPBS,50%glycerol,and0.01%sodiumazide.Pleasestoretheantibodyat-20°C.Avoidrepeatedfreeze/thawcycles.Antibodyisstablefor12monthsfromdateofreconstitution.Equilibratetheantibodytoroomtemperaturefor2minutesandmixbygentlypipettingbeforeuse.**Researchpurposesonly.Theproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals.

Immunology Consultants Laboratory, Inc.作为一家咨询公司，成立于 36 年前，在奥斯威戈湖由 Dr. Gerrie A. Leslie 博士创立。ICL 主要功能是协助定位免疫试剂以及帮助客户编写产品简介。目前位于俄勒冈州波特兰的 ICL 已发展成为一家致力于生产高质量的一级和二级抗体，抗原，以及免疫测定等服务。